Cronobacter Support
05-06-2009, 09:01 AM
1st International Conference on Cronobacter Poster Abstract 19
Comparison of Biolog GN2, BBL Crystal ENF, RapID ONE and Rapid 32E identification systems for the identification of Cronobacter (Enterobacter sakazakii).
Cronobacter is a newly proposed genus to encompass the different species referred to as Enterobacter sakazakii. These organisms have been associated with rare cases of neonatal infections. The correct identification of these species is important to infant food manufacturers as well as clinicians. The separate species within Cronobacter are as yet not represented in many identification databases. Three other recently described Enterobacter species, E. helveticus, E. turicensis and E. pulveris, that are found in the same environmental niches as Cronobacter and which represent a challenge to current isolation and conformation schemes for Cronobacter are also as yet not identifiable using many commercial systems. This study compares the performance of several identification systems for the confirmation of Cronobacter isolates and investigates the potential of the galleries to distinguish the different novel species. Strains comprised 60 Cronobacter isolates, covering the phenotypic and genetic variability of this genus, and 30 other Enterobacteriacae. Biolog GN2, BBL Crystal, RapID ONE and rapid 32E galleries were inoculated and incubated according to the manufacturer’s instructions. Biolog GN2 Microplates and BBL Crystal galleries read spectrophotometrically using the prescribed automated analyzers. The RapID ONE and rapid 32E galleries were read manually. The Biolog GN2 Microplates gave the best performance for the identification of Cronobacter species, also there is more potential for the database associated with this gallery to be amended in order to distinguish the different species. Amendments to the respective databases used for the interpretation of the results obtained from commercial galleries may improve the accuracy of these kits for identification of Cronobacter species. Clinicians and food manufacturers need to be aware of the limitations of some biochemical based identification systems for the confirmation of Cronobacter isolates.
Brendan Healy1, Angelika Lehner2, Jadwiga Goetz3, Séamus Fanning1, Roger Stephan2, Han Joosten3 and Carol Iversen1
1 Centre for Food Safety, UCD Veterinary Sciences Centre, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.2Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zürich, CH-8057, Zürich, Switzerland. 3Quality and Safety Department, Nestlé Research Centre, Vers-chez-les-Blanc, CH-1000 Lausanne, Switzerland.
Comparison of Biolog GN2, BBL Crystal ENF, RapID ONE and Rapid 32E identification systems for the identification of Cronobacter (Enterobacter sakazakii).
Cronobacter is a newly proposed genus to encompass the different species referred to as Enterobacter sakazakii. These organisms have been associated with rare cases of neonatal infections. The correct identification of these species is important to infant food manufacturers as well as clinicians. The separate species within Cronobacter are as yet not represented in many identification databases. Three other recently described Enterobacter species, E. helveticus, E. turicensis and E. pulveris, that are found in the same environmental niches as Cronobacter and which represent a challenge to current isolation and conformation schemes for Cronobacter are also as yet not identifiable using many commercial systems. This study compares the performance of several identification systems for the confirmation of Cronobacter isolates and investigates the potential of the galleries to distinguish the different novel species. Strains comprised 60 Cronobacter isolates, covering the phenotypic and genetic variability of this genus, and 30 other Enterobacteriacae. Biolog GN2, BBL Crystal, RapID ONE and rapid 32E galleries were inoculated and incubated according to the manufacturer’s instructions. Biolog GN2 Microplates and BBL Crystal galleries read spectrophotometrically using the prescribed automated analyzers. The RapID ONE and rapid 32E galleries were read manually. The Biolog GN2 Microplates gave the best performance for the identification of Cronobacter species, also there is more potential for the database associated with this gallery to be amended in order to distinguish the different species. Amendments to the respective databases used for the interpretation of the results obtained from commercial galleries may improve the accuracy of these kits for identification of Cronobacter species. Clinicians and food manufacturers need to be aware of the limitations of some biochemical based identification systems for the confirmation of Cronobacter isolates.
Brendan Healy1, Angelika Lehner2, Jadwiga Goetz3, Séamus Fanning1, Roger Stephan2, Han Joosten3 and Carol Iversen1
1 Centre for Food Safety, UCD Veterinary Sciences Centre, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.2Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zürich, CH-8057, Zürich, Switzerland. 3Quality and Safety Department, Nestlé Research Centre, Vers-chez-les-Blanc, CH-1000 Lausanne, Switzerland.