Cronobacter Support
04-29-2009, 01:22 PM
1st International Conference on Cronobacter Poster Abstract 13
Cronobacter (Enterobacter) sakazakii, Enterobacteriaceae and aerobic plate count in raw and pasteurized milk
This study was carried out to detect the presence of Cronobacter spp. (Enterobacter sakazakii), other Enterobacteriaceae and the general microbial population in raw and pasteurized milk. Total plate count (TPC) was carried out on the samples obtained from restaurant (KJG), market (FS1) and farms (ST and BL). Brilliance Enterobacter sakazakii chromogenic agar (DFI formulation) was used in isolating and detecting Enterobacteriaceae and Cronobacter spp.. Biochemical tests (Microgen GN-ID and Rapid One System) were used for the confirmation of presumptive Cronobacter isolates. Overall, results for raw milk at first and second sampling, ST first sampling contained the highest microbial load (9.4 log cfu/mL) while FS1 second sample contained the lowest microbial load at 6.4 log cfu/mL. For pasteurized milk at first and second sampling, KJG second sampling contained the highest microbial load at 7.4 log cfu/mL while FS1 first sampling contained the lowest microbial load at 5.3 log cfu/ml The TPC of pasteurized milk samples did not comply with the microbiological standards (< 100,000 cfu/ml) set by both FDA and Malaysian Food Act 1983. Cronobacter was detected in 63% (n=16) of raw milk samples and 13% (n=16) of pasteurized milk samples. One strain that was identified as ‘E. sakazakii’ using Microgen GN-ID profile was also identified as ’E. sakazakii’ using Rapid One System profile but with a higher % of probability. Enterobacteriaceae and other bacteria such as E. asburiae, Proteus vulgaris, and Xanthomonas malthophilia were also identified in the raw milk and pasteurized milk samples.
Norrakiah Abdullah Sani, Noorzatul Shuhada Md Zaini and Lim Yen Yi
Food Science Programme, School of Chemical Sciences and Food Technology, Faculty of Science and Technology, National University of Malaysia, 43600, UKM Bangi, Selangor, Malaysia.
Cronobacter (Enterobacter) sakazakii, Enterobacteriaceae and aerobic plate count in raw and pasteurized milk
This study was carried out to detect the presence of Cronobacter spp. (Enterobacter sakazakii), other Enterobacteriaceae and the general microbial population in raw and pasteurized milk. Total plate count (TPC) was carried out on the samples obtained from restaurant (KJG), market (FS1) and farms (ST and BL). Brilliance Enterobacter sakazakii chromogenic agar (DFI formulation) was used in isolating and detecting Enterobacteriaceae and Cronobacter spp.. Biochemical tests (Microgen GN-ID and Rapid One System) were used for the confirmation of presumptive Cronobacter isolates. Overall, results for raw milk at first and second sampling, ST first sampling contained the highest microbial load (9.4 log cfu/mL) while FS1 second sample contained the lowest microbial load at 6.4 log cfu/mL. For pasteurized milk at first and second sampling, KJG second sampling contained the highest microbial load at 7.4 log cfu/mL while FS1 first sampling contained the lowest microbial load at 5.3 log cfu/ml The TPC of pasteurized milk samples did not comply with the microbiological standards (< 100,000 cfu/ml) set by both FDA and Malaysian Food Act 1983. Cronobacter was detected in 63% (n=16) of raw milk samples and 13% (n=16) of pasteurized milk samples. One strain that was identified as ‘E. sakazakii’ using Microgen GN-ID profile was also identified as ’E. sakazakii’ using Rapid One System profile but with a higher % of probability. Enterobacteriaceae and other bacteria such as E. asburiae, Proteus vulgaris, and Xanthomonas malthophilia were also identified in the raw milk and pasteurized milk samples.
Norrakiah Abdullah Sani, Noorzatul Shuhada Md Zaini and Lim Yen Yi
Food Science Programme, School of Chemical Sciences and Food Technology, Faculty of Science and Technology, National University of Malaysia, 43600, UKM Bangi, Selangor, Malaysia.