Cronobacter Support
04-29-2009, 01:20 PM
1st International Conference on Cronobacter Poster Abstract 12
International survey for Cronobacter and related organisms in infant foods and formulas
In response to the FAO/WHO (2008) call for microbiological data concerning follow up formula, 8 laboratories in Brazil, Indonesia, Jordan, Korea, Malaysia, Portugal, and UK participated in surveying for Cronobacter and related organisms in their local infant formula and foods. The products were categorised according to their principle ingredients, and reconstitution instructions were collated. A total of 262 products were analysed using a standardised procedure of pre-enrichment of a 25g sample in 225 ml Buffered Peptone Water (BPW), followed by enrichment in Enterobacteriaceae Enrichment (EE) broth, plating on chromogenic Cronobacter agar; Druggan-Forsythe-Iversen agar (DFI formulation) and presumptive identification with ID32E. Presumptive Cronobacter isolates were identified using 16S DNA sequence analysis. Aerobic plate counts (APC) of the products were also determined. Thirteen samples had APC >105 cfu/g, 3 of which contained probiotic cultures. Cronobacter spp. were isolated from 36 products; 3/89 (3%) follow on formulas (as defined by Codex Alimentarius Commission), and 24/170 (14%) infant foods and drinks. Cronobacter spp. were less prevalent in follow up formula, than other foods given to infants over the same age range. Nevertheless, the later products are less likely to be subject to temperature abuse with an increase in bacterial load and risk of infection. A range of other bacteria were also isolated, including Acinetobacter baumanii, Enterobacter.cloacae, Klebsiella pneumonia, Citrobacter freundii, Serratia ficaria and Pseudomonas aeruginosa. There was significant variation in the reconstitution instructions for follow up formulas including the using water at temperatures which would enable bacterial growth. Additionally, the definition of follow up formula varied between countries.
Jessica Chapa, Philip Jacksona, Rosana Siqueirab, Nelma Gasparc, Célia Quintasc, Jon Parkd, Tareq Osmailie, Reyad Shakere, Ziad Jaradatf, Sri Harminda P. Hartantyog, Norrakiah Abdullah Sanig, Sri Estuningsihh, and Steve Forsythea
a School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham, UK. NG11 8NS. b Food Technology Institute, 2880 Brazil Ave, Campinas 13070-178, Brazil. c University of the Algarve, Engenharia Alimentar, Campus da Penha, 8000-118 Faro, Portugal. d Department of Food Science and Biotechnology, College of Engineering, Kyungwon University, Songnam, Kyonggi-do 461-701, South Korea. e Department of Nutrition and Food Technology, Faculty of Agriculture, Jordan University of Science and Technology, P.O. Box (3030), Irbid-22110, Jordan. f Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, P. O Box 3030, Irbid-22110, Jordan. g Food Science Programme, School of Chemical Sciences and Food Technology, Faculty of Science and Technology, National University of Malaysia, 43600 UKM Bangi, Selangor, Malaysia. h Faculty of Veterinary Medicine, Bogor Agricultural University, Indonesia.
International survey for Cronobacter and related organisms in infant foods and formulas
In response to the FAO/WHO (2008) call for microbiological data concerning follow up formula, 8 laboratories in Brazil, Indonesia, Jordan, Korea, Malaysia, Portugal, and UK participated in surveying for Cronobacter and related organisms in their local infant formula and foods. The products were categorised according to their principle ingredients, and reconstitution instructions were collated. A total of 262 products were analysed using a standardised procedure of pre-enrichment of a 25g sample in 225 ml Buffered Peptone Water (BPW), followed by enrichment in Enterobacteriaceae Enrichment (EE) broth, plating on chromogenic Cronobacter agar; Druggan-Forsythe-Iversen agar (DFI formulation) and presumptive identification with ID32E. Presumptive Cronobacter isolates were identified using 16S DNA sequence analysis. Aerobic plate counts (APC) of the products were also determined. Thirteen samples had APC >105 cfu/g, 3 of which contained probiotic cultures. Cronobacter spp. were isolated from 36 products; 3/89 (3%) follow on formulas (as defined by Codex Alimentarius Commission), and 24/170 (14%) infant foods and drinks. Cronobacter spp. were less prevalent in follow up formula, than other foods given to infants over the same age range. Nevertheless, the later products are less likely to be subject to temperature abuse with an increase in bacterial load and risk of infection. A range of other bacteria were also isolated, including Acinetobacter baumanii, Enterobacter.cloacae, Klebsiella pneumonia, Citrobacter freundii, Serratia ficaria and Pseudomonas aeruginosa. There was significant variation in the reconstitution instructions for follow up formulas including the using water at temperatures which would enable bacterial growth. Additionally, the definition of follow up formula varied between countries.
Jessica Chapa, Philip Jacksona, Rosana Siqueirab, Nelma Gasparc, Célia Quintasc, Jon Parkd, Tareq Osmailie, Reyad Shakere, Ziad Jaradatf, Sri Harminda P. Hartantyog, Norrakiah Abdullah Sanig, Sri Estuningsihh, and Steve Forsythea
a School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham, UK. NG11 8NS. b Food Technology Institute, 2880 Brazil Ave, Campinas 13070-178, Brazil. c University of the Algarve, Engenharia Alimentar, Campus da Penha, 8000-118 Faro, Portugal. d Department of Food Science and Biotechnology, College of Engineering, Kyungwon University, Songnam, Kyonggi-do 461-701, South Korea. e Department of Nutrition and Food Technology, Faculty of Agriculture, Jordan University of Science and Technology, P.O. Box (3030), Irbid-22110, Jordan. f Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, P. O Box 3030, Irbid-22110, Jordan. g Food Science Programme, School of Chemical Sciences and Food Technology, Faculty of Science and Technology, National University of Malaysia, 43600 UKM Bangi, Selangor, Malaysia. h Faculty of Veterinary Medicine, Bogor Agricultural University, Indonesia.