Cronobacter Support
04-27-2009, 10:49 AM
1st International Conference on Cronobacter Poster Abstract 9
Molecular analysis of the Enterobacter sakazakii O-antigen gene locus
Nucleotide polymorphism associated with the O-antigen encoding locus, rfb, in Enterobacter sakazakii was determined by PCR-RFLP analysis. Based on the analysis of these DNA profiles, 12 unique banding patterns were detected amongst a collection of 62 strains from diverse origins. Two common profiles were identified and designated as serotypes O:1 and O:2. DNA sequencing of the 12,500-bp region, flanked by galF and gnd, identified 11 open reading frames, all with the same transcriptional direction. Analysis of the proximal region of both sequences demonstrated remarkable heterogeneity. A polymerase chain reaction (PCR) assay targeting genes specific for the two prominent serotypes was developed, and applied for the identification of these strains recovered from food, environmental and clinical samples.
Niall Mullane1,2, Brendan Healy1,2, Peadar O'Gaora3, Jarlath Nally2, Carol Iversen1,2,4, Paul Whyte1,2, Patrick Wall5 and Séamus Fanning1,2
1Centres for Food Safety and Food-borne Zoonomics, UCD Veterinary Sciences Centre, 2School of Agriculture, Food Science & Veterinary Medicine, 3Conway Institute of Biomolecular & Biomedical Research, 5School of Public Health & Population Sciences, University College Dublin, Belfield, Dublin 4, Ireland and 4Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zürich, CH-8057, Zürich, Switzerland.
Molecular analysis of the Enterobacter sakazakii O-antigen gene locus
Nucleotide polymorphism associated with the O-antigen encoding locus, rfb, in Enterobacter sakazakii was determined by PCR-RFLP analysis. Based on the analysis of these DNA profiles, 12 unique banding patterns were detected amongst a collection of 62 strains from diverse origins. Two common profiles were identified and designated as serotypes O:1 and O:2. DNA sequencing of the 12,500-bp region, flanked by galF and gnd, identified 11 open reading frames, all with the same transcriptional direction. Analysis of the proximal region of both sequences demonstrated remarkable heterogeneity. A polymerase chain reaction (PCR) assay targeting genes specific for the two prominent serotypes was developed, and applied for the identification of these strains recovered from food, environmental and clinical samples.
Niall Mullane1,2, Brendan Healy1,2, Peadar O'Gaora3, Jarlath Nally2, Carol Iversen1,2,4, Paul Whyte1,2, Patrick Wall5 and Séamus Fanning1,2
1Centres for Food Safety and Food-borne Zoonomics, UCD Veterinary Sciences Centre, 2School of Agriculture, Food Science & Veterinary Medicine, 3Conway Institute of Biomolecular & Biomedical Research, 5School of Public Health & Population Sciences, University College Dublin, Belfield, Dublin 4, Ireland and 4Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zürich, CH-8057, Zürich, Switzerland.