Cronobacter Support
08-27-2009, 10:06 AM
Matrix MicroScience has launched a novel and rapid method for the detection of Enterobacter sakazakii and other Enterobacteriaceae in infant feed formulas and milk powders. The method utilises cationic (positively charged) paramagnetic particles.
The key features offered by the new method are:
Reduction in time to result from the current 4+ days taken by the FDA method to a new test time of 23 hours, from sampling to final colonies on selective agars.
Increase in sample throughput and the opportunity to increase the size of the sample tested—up to 500 g, as opposed to the current test limit of 100 g. In this case, the Pathatrix pooling strategy is used.
Pathatrix Pooling - Infant Formula on VRBGA
http://www.rapidmicrobiology.com/news/142h5A.JPG
Compatibility with the all currently available end-point detection systems, e.g. selective agars, PCR, ELISA, colorimetric tests, etc.
Simplicity of use and implementation, and using the same techniques and equipment that are used on the Pathatrix system to detect other pathogens, e.g. Salmonella, Listeria, E. coli O157, MAP, viruses, etc.
In addition to the above, the unique use of cationic (positively charged) particles offers the added benefit that this approach also captures other Enterobacteriaceae including Citrobacter diversus, C. freundii, E. cloacae, Klebsiella oxytoca, K. pneumoniae, K. aerogenes, Serratia marcescens, Salmonella enteritidis,S. typhimurium and S. poona, so offering the possibility to conduct an initial Enterobacteriaceae screen followed by specific tests for Enterobacter sakazakii or Salmonella spp.
Joint Capture of S.enteritidis + E.sakazakii
http://www.rapidmicrobiology.com/news/142h5B.JPG
Spike Levels - S.enteritidis = 4cfu/5g, E.sakazakii = 2cfu/5g
This simple and cost-effective method has considerable promise for the early detection of E. sakazakii and other Enterobacteriaceae in infant feed formulas.
Posted from rapidmicrobiology.com
The key features offered by the new method are:
Reduction in time to result from the current 4+ days taken by the FDA method to a new test time of 23 hours, from sampling to final colonies on selective agars.
Increase in sample throughput and the opportunity to increase the size of the sample tested—up to 500 g, as opposed to the current test limit of 100 g. In this case, the Pathatrix pooling strategy is used.
Pathatrix Pooling - Infant Formula on VRBGA
http://www.rapidmicrobiology.com/news/142h5A.JPG
Compatibility with the all currently available end-point detection systems, e.g. selective agars, PCR, ELISA, colorimetric tests, etc.
Simplicity of use and implementation, and using the same techniques and equipment that are used on the Pathatrix system to detect other pathogens, e.g. Salmonella, Listeria, E. coli O157, MAP, viruses, etc.
In addition to the above, the unique use of cationic (positively charged) particles offers the added benefit that this approach also captures other Enterobacteriaceae including Citrobacter diversus, C. freundii, E. cloacae, Klebsiella oxytoca, K. pneumoniae, K. aerogenes, Serratia marcescens, Salmonella enteritidis,S. typhimurium and S. poona, so offering the possibility to conduct an initial Enterobacteriaceae screen followed by specific tests for Enterobacter sakazakii or Salmonella spp.
Joint Capture of S.enteritidis + E.sakazakii
http://www.rapidmicrobiology.com/news/142h5B.JPG
Spike Levels - S.enteritidis = 4cfu/5g, E.sakazakii = 2cfu/5g
This simple and cost-effective method has considerable promise for the early detection of E. sakazakii and other Enterobacteriaceae in infant feed formulas.
Posted from rapidmicrobiology.com