Cronobacter Support
05-22-2009, 06:48 PM
1st International Conference on Cronobacter Poster Abstract 48
Inactivation of Cronobacter spp (Enterobacter sakazakii) in infant formula using lactic acid and copper sulphate
Enterobacter sakazakii (Cronobacter spp) is an opportunistic pathogen that poses a potential health risk to neonates. It is a known contaminant of infant formula milk (IFM), and has been associated with cases of necrotizing enterocolitis and infant meningitis. The use of natural antimicrobials has not been fully explored as an option for the control of the pathogen. The purpose of this study was to investigate the use of lactic acid and copper sulfate, as natural antimicrobials against E. sakazakii in IFM. Re-hydrated infant formula milk (RIFM) and powdered infant formula milk (PIFM) were inoculated with a five-strain mixture of E. sakazakii, and the samples were treated with copper and lactic acid, alone and in combination. The use of a copper sulfate at a concentration of 50 ppm and lactic acid at 0.2% v/v had a slight but remarkable effect on the growth of E. sakazakii, the combination of both agents resulted in a complete inhibition of growth in PIFM after 2 hours. In RIFM, the combination resulted in a 3-log reduction after 2 hours, and complete inhibition of growth after 6 hours. The results indicate that the combination of lactic acid and copper sulfate could be used for the control of E. sakazakii contamination in IFM. The use of small doses of natural antimicrobials can be beneficial to both the food industry and the consumers. It could also improve food safety, and reduce the need for artificial chemical preservatives.
Murad A. Al-Holy1, Luis F. Castro2, Hamzah M. Al-Qadiri3 and Barbara A. Rasco2
1Department of Clinical Nutrition and Dietetics, Faculty of Allied Health Sciences, Hashemite University, Zarqa-Jordan. 2School of Food Science, Box 646376, Washington State University, Pullman, WA, USA. 3Department of Nutrition and Food Technology, Faculty of Agriculture, University of Jordan, Amman, Jordan.
Inactivation of Cronobacter spp (Enterobacter sakazakii) in infant formula using lactic acid and copper sulphate
Enterobacter sakazakii (Cronobacter spp) is an opportunistic pathogen that poses a potential health risk to neonates. It is a known contaminant of infant formula milk (IFM), and has been associated with cases of necrotizing enterocolitis and infant meningitis. The use of natural antimicrobials has not been fully explored as an option for the control of the pathogen. The purpose of this study was to investigate the use of lactic acid and copper sulfate, as natural antimicrobials against E. sakazakii in IFM. Re-hydrated infant formula milk (RIFM) and powdered infant formula milk (PIFM) were inoculated with a five-strain mixture of E. sakazakii, and the samples were treated with copper and lactic acid, alone and in combination. The use of a copper sulfate at a concentration of 50 ppm and lactic acid at 0.2% v/v had a slight but remarkable effect on the growth of E. sakazakii, the combination of both agents resulted in a complete inhibition of growth in PIFM after 2 hours. In RIFM, the combination resulted in a 3-log reduction after 2 hours, and complete inhibition of growth after 6 hours. The results indicate that the combination of lactic acid and copper sulfate could be used for the control of E. sakazakii contamination in IFM. The use of small doses of natural antimicrobials can be beneficial to both the food industry and the consumers. It could also improve food safety, and reduce the need for artificial chemical preservatives.
Murad A. Al-Holy1, Luis F. Castro2, Hamzah M. Al-Qadiri3 and Barbara A. Rasco2
1Department of Clinical Nutrition and Dietetics, Faculty of Allied Health Sciences, Hashemite University, Zarqa-Jordan. 2School of Food Science, Box 646376, Washington State University, Pullman, WA, USA. 3Department of Nutrition and Food Technology, Faculty of Agriculture, University of Jordan, Amman, Jordan.