Cronobacter Support
05-15-2009, 06:42 PM
1st International Conference on Cronobacter Poster Abstract 31
Complete inactivation and removal of attached cells of Cronobacter spp. using Hydrofem electrolysed water (‘Anolyte’).
Cronobacter spp. comprise a family of bacteria, many of which are pathogens associated with meningitis, septicemia, and necrotizing enterocolitis, primarily among low birth-weight and premature infants. Powdered infant formula has been epidemiologically linked with infection. Attachment and biofilm formation by Cronobacter spp. in the production of PIF may present a source of recontamination of final product. The aim of this work was to demonstrate the effectiveness of Hydrofem electrolysed water (or ‘Anolyte’) for inactivation and removal of attached cells of Cronobacter spp. fifteen strains (including clinical isolates from different sources) were shown to attach to stainless steel coupons when incubated in Nutrient Broth for 3 days. Inactivation of planktonic cells was tested by direct inoculation into ‘Anolyte’, or by spraying culture that was spresd on surfaces. Generally, the number of attached cells was about 3.5 log CFU/cm2, although one strain attached at a rate of 5.6 log CFU/cm2. A subsequent 5 minute ‘Anolyte’ treatment was sufficient to completely remove and inactivate 10 of these strains. Inoculation of cells into ‘Anolyte’ caused immediate and complete inactivation. Cultures spread on different surfaces, including glass, a rough surface ceramic tile, a smooth surface ceramic tile and a laboratory bench were completely inactivated by spraying the surface with ‘Anolyte’ (with a residence time of 2 min.) In conclusion, ‘Anolyte’ has the potential to contribute to the overall strategy for control of Cronobacter spp. on surfaces.
Edward M. Fox1, Edmond O’Reilly2 and Kieran N. Jordan1.
1 Teagasc, Moorepark Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland and 2 Trustwater Ltd., Gurtnafleur Business Park, Clonmel, Co. Tipperary, Ireland.
Complete inactivation and removal of attached cells of Cronobacter spp. using Hydrofem electrolysed water (‘Anolyte’).
Cronobacter spp. comprise a family of bacteria, many of which are pathogens associated with meningitis, septicemia, and necrotizing enterocolitis, primarily among low birth-weight and premature infants. Powdered infant formula has been epidemiologically linked with infection. Attachment and biofilm formation by Cronobacter spp. in the production of PIF may present a source of recontamination of final product. The aim of this work was to demonstrate the effectiveness of Hydrofem electrolysed water (or ‘Anolyte’) for inactivation and removal of attached cells of Cronobacter spp. fifteen strains (including clinical isolates from different sources) were shown to attach to stainless steel coupons when incubated in Nutrient Broth for 3 days. Inactivation of planktonic cells was tested by direct inoculation into ‘Anolyte’, or by spraying culture that was spresd on surfaces. Generally, the number of attached cells was about 3.5 log CFU/cm2, although one strain attached at a rate of 5.6 log CFU/cm2. A subsequent 5 minute ‘Anolyte’ treatment was sufficient to completely remove and inactivate 10 of these strains. Inoculation of cells into ‘Anolyte’ caused immediate and complete inactivation. Cultures spread on different surfaces, including glass, a rough surface ceramic tile, a smooth surface ceramic tile and a laboratory bench were completely inactivated by spraying the surface with ‘Anolyte’ (with a residence time of 2 min.) In conclusion, ‘Anolyte’ has the potential to contribute to the overall strategy for control of Cronobacter spp. on surfaces.
Edward M. Fox1, Edmond O’Reilly2 and Kieran N. Jordan1.
1 Teagasc, Moorepark Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland and 2 Trustwater Ltd., Gurtnafleur Business Park, Clonmel, Co. Tipperary, Ireland.