Cronobacter Support
05-12-2009, 07:16 PM
1st International Conference on Cronobacter Poster Abstract 30
Incidence of Cronobacter sakazakii in powdered-milk-based infant formula in Dubai (United Arab Emirates).
As an emerging pathogen C. sakazakii, received much attention after April 2002 when the US - FDA issued an alert to health care professionals regarding the risk associated with C. sakazakii infection among neonates fed milk based powdered infant formulas. C. sakazakii infection was rare but, life threatening cause of neonatal meningitis, sepsis and severe neurological impairment. In general fatality rate varies from 40-80% among newborns diagnosed with this type of severe infection. The United Arab Emirates, like other Gulf countries largely depends on imported foods from around 100 countries around the world. Part of it consumed locally and the majority 75% re-exported to other countries in the Middle East and Africa. Surveys were conducted annually though the period 2003 to 2008 comprising a total of 735 samples with a total of 31 positive isolates for the organism. Isolates were maintained in Cryobank system and subjected to further confirmation and characterization studies. The organism was found to be resistant to 4 antibiotics out of 21 tested. Those are Cefoxitin, Cephalothin, Ampicillin and Amoxicillin. Generation times of the organism were calculated at different temperatures, 25, 30 and 45°C and was found to be 36, 24.6 and 20.4 minutes respectively. Survival of the organism in dry powder was studied and the population showed stability in counts during the 34 weeks experimental period with minimal fluctuations resulting from inoculums differences. Growth at different pH was studied and the organism was shown to be inhibited at pH 2.5, 3.5 and was able to grow at, 4.5, 5.5, 6.5, 7.5 and 8.5. Susceptibility of the organism to different disinfectants that are used in food manufacturing premises as well as for cleaning infant feeding bottles were studied and the organism was completely destructed by all of 5 types used in the experiment. The risk of infection form an infant formula sold in Dubai was calculated and described to be very minor as well as risk was decreasing on annual basis from 2003 up to now and that is due to awareness of connected parties, major changes in code hygiene and infant formula processing development in reaction to the problem.
Hamid Mohamed Ibrahim
Dubai Municipality, United Arab Emirates (U. A. E.)
Incidence of Cronobacter sakazakii in powdered-milk-based infant formula in Dubai (United Arab Emirates).
As an emerging pathogen C. sakazakii, received much attention after April 2002 when the US - FDA issued an alert to health care professionals regarding the risk associated with C. sakazakii infection among neonates fed milk based powdered infant formulas. C. sakazakii infection was rare but, life threatening cause of neonatal meningitis, sepsis and severe neurological impairment. In general fatality rate varies from 40-80% among newborns diagnosed with this type of severe infection. The United Arab Emirates, like other Gulf countries largely depends on imported foods from around 100 countries around the world. Part of it consumed locally and the majority 75% re-exported to other countries in the Middle East and Africa. Surveys were conducted annually though the period 2003 to 2008 comprising a total of 735 samples with a total of 31 positive isolates for the organism. Isolates were maintained in Cryobank system and subjected to further confirmation and characterization studies. The organism was found to be resistant to 4 antibiotics out of 21 tested. Those are Cefoxitin, Cephalothin, Ampicillin and Amoxicillin. Generation times of the organism were calculated at different temperatures, 25, 30 and 45°C and was found to be 36, 24.6 and 20.4 minutes respectively. Survival of the organism in dry powder was studied and the population showed stability in counts during the 34 weeks experimental period with minimal fluctuations resulting from inoculums differences. Growth at different pH was studied and the organism was shown to be inhibited at pH 2.5, 3.5 and was able to grow at, 4.5, 5.5, 6.5, 7.5 and 8.5. Susceptibility of the organism to different disinfectants that are used in food manufacturing premises as well as for cleaning infant feeding bottles were studied and the organism was completely destructed by all of 5 types used in the experiment. The risk of infection form an infant formula sold in Dubai was calculated and described to be very minor as well as risk was decreasing on annual basis from 2003 up to now and that is due to awareness of connected parties, major changes in code hygiene and infant formula processing development in reaction to the problem.
Hamid Mohamed Ibrahim
Dubai Municipality, United Arab Emirates (U. A. E.)